how do you create trubreeds from hybrids ?

Discussion in 'Cannabis Breeding and Seed Strains' started by tiedye420, Mar 20, 2005.

  1. tiedye420

    tiedye420 Member

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    MEAN or GECK or anybreeder knowledgeables!!
    If i take the f2 version of a multiple hybrid
    And i seperate the phenotypes, selectivly breeding each pheno within itself.
    Can i create a trubreeding strain this way?
    Or rather 7 trubreeding strains, as there are 7 phenotypes.{mexican indica, afghani 1. afghani 2, nepalese hashplant, chocolate columbian, hawaiian, and another pheno that might be ruderalis.REAL small- Not much smell- but a one bud pheno}
    Im doing this in the f2 version, Im open pollenating the f3 versions- all 7 of them seperately.
    The choice male from each pheno will have a seperate bottle of pollen from it.
    the males from each pheno will be blended into a bottle labled accordingly.
    How many generations do I have to go with this to have truebreeds?
    Is it f5?
     
  2. meangreen

    meangreen Senior Member

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    Good luck,that could take ya years to accomplish if you ever could.Too many strains to even try and create a stable breed!
     
  3. meangreen

    meangreen Senior Member

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    Why,is what I ask?You have had hermie probs with some of those,it seems pointless to me,but to each there own!
     
  4. tiedye420

    tiedye420 Member

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    bah humbug
    they are seperated already into phenos,
    how many generations from f3 to truebreed are needed?
    I have quite a few years left on the planet been at one side of this cross 9 years already.
    But im not talking about the hybrids man
    Im talking about trubreeding the various phenos of an f2, seperately.
    what are the drawbacks, other than an obvious small genepool thing.
     
  5. tiedye420

    tiedye420 Member

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    the columbian pheno is suspect for causing hermie traits, i figure to weed it out and rebuild a few different versions without the chocolate.
    I may have outcrossed the hermie traits as well, with the humbolt cross/selective breeding to f2 from non hermie parents.
    Furthurmore
    I learned to inbreed hermie traits- now i need to learn to outbreed them- hopefully without recessing the genes- but removing, Or changing it.
     
  6. WayfaringStranger

    WayfaringStranger Corporate Slave #34

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    i would doubt it be possible. if it were, i doubt it would be possible to know if youve succeeded. if there were only 2 or 3 genes present in the whole genome, you might have a shot at knowing if you were succesfull, it just sounds like a headache that no amount of THC will subside.
     
  7. tiedye420

    tiedye420 Member

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    the only strain low in thc would be the ruderalis- which is what causes the hummer to autoflower im sure.

    This is really the only thing that interests me anymore, breeding.
    ya gotta grow to get the seeds man.
     
  8. tiedye420

    tiedye420 Member

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    Hi wayfaring how's it going?
    do you understand me at all?
    i mean seven stable breed
    not a mixed up hybrid
    i know how to stabalize it.
    Im talking about seperating the phenos of all seven strains
    A:F2 selectively breeding within each pheno ,from the varieties seperatly creating seven f3 strains.
    B:taking seven different strains, and open pollenating 50 or 100 seeds from each phenotype created in the f2 version.Seperately.
    F4 is trubreed?
    or do I gotta open pollenate twice?
    and take it to f5.???????????????????????????????????
    TELL ME NOW OR ILL PUSH THE RED BUTTON AND KILL US ALL.
    how many generations do i take it from f2 to truebreed?
    FORGET HOW LONG OR HOW HARD_ MOOT POINT- I DO NOT CARE
    open pollenation and selective breeding was how land race was created-n you cannot destroy landrace with your hybrids, I will remove them all from every genepool in the world if i get my way- only landrace named after village and or country.

    You must be asssimilated you must be assimilated you must be assimilated.
    OH MY GOSH i think i spent a little too much time researching orgNICS.
    ADVANCE NUTES KICKS ASS.
    bUT YOU GOTTA SPEND AROUND $350 TO ADHERE TO THE FORMULA MIXING CHART, with the vodoo juice and piranha and all they reccomend for cannabis .
     
  9. tiedye420

    tiedye420 Member

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    but robert clarke says:

    Occasionally hybrid offspring will prove inferior to both parents, but the first generation may still contain recessive genes for a favorable characteristic seen in a parent if the parent was homozygous for that trait. First generation (F1) hybrids are therefore inbred to allow recessive genes to recombine and express the desired parental trait. Many breeders stop with the first cross and never realize the genetic potential of their strain. They fail to produce an F2 generation by crossing or self-pollinating F1 offspring. Since most domestic Cannabis strains are F1 hybrids for many characteristics, great diversity and recessive recombination can result from inbreeding domestic hybrid strains. In this way the breeding of the F1 hybrids has already been accomplished, and a year is saved by going directly to F2 hybrids. These F2 hybrids are more likely to express recessive parental traits. From the F2 hybrid generation selections can be made for parents which are used to start new true-breeding strains. Indeed, F2 hybrids might appear with more extreme characteristics than either of the P~ parents. (For example, P1 high-THC X P1 low-THC yields F1 hybrids of intermediate THC content. Selfing the F1 yields F2 hybrids, of both P1 [high and low THC] phenotypes, inter mediate F1 phenotypes, and extra-high THC as well as extra-low THC phenotypes.)

    Also, as a result of gene recombination, F1 hybrids are not true-breeding and must be reproduced from the original parental strains. When breeders create hybrids they try to produce enough seeds to last for several successive years of cultivation, After initial field tests, undesirable hybrid seeds are destroyed and desirable hybrid seeds stored for later use. If hybrids are to be reproduced, a clone is saved from each parental plant to preserve original parental genes.

    Back-crossing is another technique used to produce offspring with reinforced parental characteristics. In this case, a cross is made between one of the F~ or subsequent offspring and either of the parents expressing the desired trait. Once again this provides a chance for recombination and possible expression of the selected parental trait. Back-crossing is a valuable way of producing new strains, but it is often difficult because Cannabis is an annual, so special care is taken to save parental stock for back-crossing the following year. Indoor lighting or greenhouses can be used to protect breeding stock from winter weather. In tropical areas plants may live outside all year. In addition to saving particular parents, a successful breeder always saves many seeds from the original P1 group that produced the valuable characteristic so that other P1 plants also exhibiting the characteristic can be grown and selected for back-crossing at a later time.
     
  10. tiedye420

    tiedye420 Member

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    I crossed two strains.
    There are it seems, six phenos to the f2 generation.
    the intermediate phenos resemble mexican indica, and ruderalis.
    the main phenos resemble the p1&2 of both strains.
    I can create truebreeds from these phenos.
    But some will be undesireable (small hashplant with low potency-ect.}
    selective breeding from this f2 gen will be interesting.
    Robert still did not point out how many generations after f2 is needed to create trubreed.
    My only question is and was from the beginning is, how many generations are needed from f2 to true.
    Im off to overgrow.
     
  11. geckopelli

    geckopelli Senior Member

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    Creating a stable strain will require a lot of back breeding.

    Breed every third generation with it's granparent generation. Inbreed the other two.

    If your lucky, you'll see a move toward uniformity in the 10th generation.

    or that's the theory, anyway!

    I had an 8th generation that seemed to have firmly divided into only two genotypes.
     
  12. tiedye420

    tiedye420 Member

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    the method im using is filial selection.
    only the P2 from the desired cross is available still.
    unles i plant my clavo of p1&p2, and f1 seeds, which are due to be regenerated this year anyways.
    but, there was a random hermie-p1 she was used to make fem. seeds.in 1998-99,there was male pollen from the columbianxhashplant{ a selected f2} was used also.Those were the only breederstock used at the time.

    I have decided the only way to backcross to p1 or p2 is to use a male from p2
    (p1 is femm. only}
    im cloning all the females, until they have budded and fruit has been analized.
    then backrossing will be posible to the stinkiest male p2 hashplant i can find
    but this is what is nessesary to trubreed my hybrid.......
    Im seeing some real nice phenos- all heavy branchers indoor.....anyspectrum.
    im seperating the 4 main phenos, inbreeding the phenos to themselves.....
    I may do selective breeding within the different phenos, according to smell, or taste, or some other desired trait.

    there are some growfaqs on overgrow, that definetly pertain to this....
    http://www.overgrow.com/growfaq/t150

    i think i need to cube them for the fastest results, from what im gathering here and there..

    thanks geck
     
  13. tiedye420

    tiedye420 Member

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    CAN SOMEONE DESIPHER THIS
    seems like selection, and cubing will get me true enough.
    if I can get 71.875% to taste and smell like hawaiian punch {stinky when cured}I'll be a happy camper. im on it man.
    from OG FAQS
    BackCrossing and Cubing

    Backcrossing is where you breed an individual (your special clone) with it's progeny. Sick in our world, but plants seem to like it

    1) Your first backcross is just a backcross.

    2) Your second backcross where you take the progeny from the first backcross and cross back to the SAME parent (grandparent now) is often called SQUARING by plant breeders.

    3) Your third backcross where you take the progency (squared) from the second backcross and cross back to the SAME parent (great grandparent now) is often called CUBING by plant breeders. You can continue the backcrossing but we just call this backcrossing. Cubing is in reference to the number three, as in 3 backcrosses

    Cubing works on the basis of mathamatical probabilities with respect to gene frequencies. The more males you use with each cross, the better the chance that your reality matches the theory. In theory, with the first backcross, 75% of your genepool will match the genepool of the P1 parent being cubed. Squaring increases this to 87.5% and cubing increases it to 93.75%. You can arrive at these numbers by taking the average between the two parents making up the cross. For instance, you start by crossing the P1 mom (100%) with and unrelated male (0%) getting 100% + 0% divided by 2 = 50%. Therefore, the offspring of this first cross are loosly thought of as being 50% like the mom. Take these and do your first backcross and you get 100% (mom) + 50% divided by 2 = 75%. And this is where we get the 75% for the first backcross. Same thing applies as you do more backcrosses. As you will see later, you can apply this same probability math to specific genes or traits, and this can have a dramatic effect on your methodology and selection methods.

    Your selection of the right males for each backcross are the crucial points for success with this technique. In each case, you could select males that contain the genes you want, or you could inadvertedly pick those individuals that carry the unwanted recessive genes. Or more likely, you could just pick individuals that are heterozygous for both genes like the P1 mom being backcrossed. The easiest way to deal with this is to start by only looking at one gene and one trait, like lets assume that flavour is determined by a single gene (in reality it's probably not). And do some punnet squares to show gene frequencies through 3 generations of backcrossing. Now lets assume that we found a special pineapple flavoured individual in our pine flavoured population that we wanted to keep. The gene causing the pineapple flavour could be dominant or recessive and the selection abilities and cubing outcome will be different in both cases.

    a) pineapple flavour is dominant.

    P = pineapple flavour and p = pine flavour

    Therefore since each individual will have two flavour genes paired up, the possible genotypes are PP, Pp, and pp. Since P is dominant, PP and Pp will express pineapple flavour while pp will exhibit pine flavour, these are their phenotypes. Now since the pineapple is a new flavour, chances are that the special individual will be heterozygous, or more specifically, Pp. Therefore, the only possible parent combination is Pp X pp with the Pp being the parent to be cubed.
     
  14. WayfaringStranger

    WayfaringStranger Corporate Slave #34

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    tiedie, you seem like the expert to me, so i'll let you do the leg work, and can i just get seeds from you?
     
  15. tiedye420

    tiedye420 Member

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    I used two IBL to start this current work.
    I am using the f2 version now.
    If i selective breed the desired pheno[the one resembling P1} from the f2
    backcross to P1 to create F3.
    F3 will have a higher % of the desired pheno.
    THEN I backcross F3 to P1 TO MAKE IT A TRUBREED.
    I just need to go to F4- as for seperating the phenos- impossible, unless i backcross, or cross to a IBL SIMILAR IN CHARACHTERISTICS.
    f4 WAS THE ANSWER.


    here are growfaqs from O.G.
    MAKING IT SIMPLE.
    An IBL (inbred line) is a genetically homogeneous strain that grows uniformly from seed.

    A hybrid is a strain made up of two genetically unlike parents, IBL or hybrid.

    When you cross two different IBL strains for the FIRST time, it is called the F1 generation. When you cross two of the same F1 hybrid (inbreed), it is called the F2 generation.

    The process of selective inbreeding must continue at least until the F4 to stabilize the recurrently selected traits. When you cross two specimens of an IBL variety, you get more of the same, because an IBL is homozygous, or true breeding for particular traits.
     
  16. meangreen

    meangreen Senior Member

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    Still like to know why you are going to all this trouble when there are hundreds of already proven stable breeds available from many seed banks that dont require a detailed breeding process,in other words what is your goal that cannot be found at a seed bank with no effort,especially with the known hermaphroditism that has been present in your garden as long as I have known ya!Seems alot more reasonable to get some known stables and make hybrids from them!
     
  17. tiedye420

    tiedye420 Member

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    HEre is the deal, where the hermies came from.
    I am breeding my best work. My original crosses the seeds are going to be f2 or s2 either feminised or not.
    But the males will not carry the hermie traits. I had I made femminised seed in 1998-99. The original hashplant was humbolt growerunion seedstock. Brought to backcross to the original- to renew the genetic composition of humbolt.
    I crosed it with the chocolate columbian in 1999. I also used pollen from a very choice male f1. THose seeds I grew a few of in 2000, some hermied out. I grew them out away from the others, to keep the hermie genes away from the other plants i had going then. THe hermie went up in flame in the wildfires- the others got greymold in the sierra foothills. I grew the third gen out in this trailer- learning how to grow indoors. third gen from the hermies that is. I Still have feminised and f2 versions of this work up the hill in storage- THE ORIGINL ONES FROM 1999!!!!!

    but it gets deeper the plants i have growing now are a f2 backcross to the hasplant which now becomes P1 and p2- cousins- a backcrosss within the saame genotype- locking in the extremely branchy trait i have been developing -----stinky breeds true for branching because of the law of recombination-
    So when i crossed the chocolate lumbo to humbolt it WAS a backcrosss...Outcroissing the hermie traits as far as i know- but if I use a male from my first seeds my first gen will have males- males with no s-1 or selved genes in them.
    Those will also be used to backcross ,squaring and cubing non- hermie traits.

    LIKE I SAID BEFORE- learn to breed in, learn to breed out, pretty soon it will be just like breathing..
    I have been reading clarke for over 20 years now- and i actually beginning to get a slight grasp on botany because of robert.

    But yeaah last year I Grew test seed from hermies L.O.L. NOw that I know a little about the indoor trip- Im bustin out my old ones- time to regenerate those babys anyhow.THEY were made in 1999.
    I have spent months trying to figure out what i did- so i can figure out what to do, but soetimes i think to much and just need to rely on instinct{backcrosssing to the humbolt - locked in the branching due to recombination- and may have outcrosed the hermie traits as well.. I'll find out soon enough...
    Only BOG seeds has plants that branch like mine bro, only BOG.
     
  18. meangreen

    meangreen Senior Member

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    I would sure like to see some pics of these when and if they come about,I wish ya the best!
     
  19. meangreen

    meangreen Senior Member

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    Here are some heavy yielding ladies!

    [​IMG]
     
  20. tiedye420

    tiedye420 Member

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    I first have to stabalize certain traits, then backcross to the humbolt, im trying to come up with a fruit punch flavor, throughout the strain- stinky-the F1 {backcross within genotype} had it ,and Im hoping for 75% with it this run. Might be a reccessive trait and only 20% or something though.time will tell.

    BUT when I Start making seeds by the thousands,you'll know im getting ready to release some soon.. L.O.L.


     
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