Best newb way to create liquid mycelium cultures for reusing syringes (and saving $$)

Seen some products but are costly. Is there a cheap and dirty (but successful) guide to taking mushrooms or colonized mycelium and putting it in a liquid culture for infinite use without agars etc?

 

A culture will never be infinite. The mycelium is the roots for the mushrooms, but instead of living off of water and soil, it lives off of food and high nutrient concentrated chemicals. SO basically it sucks the life out of things. Unless you have a fountain of youth, they gonna die

Dirty+successful+Mushrooms in the same sentence? Replace dirty with clean then you can continue.

You are really new to this huh?

 

yep

 

Please don't listen to aesthetic... is the local wellspring of shitty information. (I honestly can't figure out if he is really confused or is intentionally trying to screw people up with bad info)


Making liquid culture is easy.

1) Add 1 level teaspoon of light corn syrup (Karo Syrup or the "no-name" store brand of Light Corn Syrup) per 100 ml of water

2) Stir until the corn syrup is throughly dissolved and pour into a canning jar.

3) Drill a small hole into the lid of the canning jar

3) Place a Tyvek filter on top ( These are 6 in x 6 in tyvek patches that were cut out of a tyvek sheet you can get at a home depot or you can get tyvek Priority Mail envelopes from your local post office for FREE.

4) Put lid on top with seal facing up and screw down lid ring

5) Add two coffee filters to the lid and secure with a sturdy rubber band

6) Cover coffee filters with tin foil and secure with a sturdy rubber band (this is done to keep water from seeping into the jars during sterilization)

7) Sterilize the jar for 30 minutes at 15 psi and let the PC depressurize on its own.

8) Last but not least remove the foil from the lids to allow the coffee filters to dry and let the jar cool overnight.

9) Innoculate inside a glove box with 1 cc of spore syringe solution or a clean piece of mycellium or mushroom tissue.

The coffee filters coupled with the tyvek work well as a barrier IMO. I find the best way to innoculate a jar is through these filters. I apply alittle Iso alcohol to the top of the jar and the coffee filters become transparent so you can line up your flame sterilized needle with the hole in the lid.. then upon removing the needle I cover the hole with a napkin soaked in Iso alcohol and rotate the coffee filters 180 degrees so the holes don't line up with the innoculation hole in the lid.... all very sterile and the alcohol evaporates in a few mintues.

10) Let sit in a dark clean draft free location at 85f for 1 week.

11) Gently swish jar to break up tissue clumps and suck up liquid culture into a clean syringe and use it to inocculate grain jars.

For pictures of the lid process see this guide: http://www.hipforums.com/newforums/showthread.php?t=416240&f=118

You can use this liquid culture to inocculate more corn syrup jars for several generations as long as you keep it clean.



I don't care for liquid culture because it is hard to spot contamination while it is in liquid. Usually it is not until you have inocculated a bunch of grain jars that they show their ugly faces. This is why agar is prefered because the cultivated fungi and contams grow out on a 2 dimensional plane so it is easy to see any molds or bacteria and seperate it from the shroom mycellium.

 

Another way to make liquid culture is to take a jar of colonized broken up grain spawn and inject 12 ccs of clean sterile water. Shake the ever living piss out of the jar to break up all mycellium and distribute it into solution. Then suck up the liquid back into the syringe and use it to shoot up other jars.


This only works with grain jars..... not pf brf jars.


I still recommend learning agar culture techniques. It will increase your productivity by 5 fold.

 

Never said it wasn't possible. The way he asked though is impossible. And a wellspring? Hardly. Dirty method with mushrooms? Thats dangerous, I probably saved someones life. Infinite mushrooms? Get real. Turning a Ricecake into a liquid substrate? Also get real.

 

Try reading what I wrote before spewing more of your garbage.

It is called the GLC tek:

http://www.shroomery.org/forums/showflat.php/Number/15308963#15308963


And now that you mention it there is a tek on making liquid culture from a brf jar that dates back to 2003.

http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek

 

Read very carefully, I know its difficult but be sure to enunciate the words when I type them Liiiiikkkkkeee thiiiiiisssss. Sometimes I do this when my mind is clouded with anger, helps me interpret peoples segments better.

But It looks like your one of the people that I cant reason with and have poor eyesight. Forgive me if you took any of what I said personally.

I know you will either not respond at all or disregard what I said or say something about my mother and they way she gets when she sees racoons.

Sooo I will leave this post up to you. But just to make it clear, YOU CANT HAVE INFINITY MUSHROOMS FROM ONE JAR! I was pointing out that you cant cut out a piece of a already cultivated ricecake and transfer it into a liquid substrate. ( I may be wrong on that one, but it doesnt seem possible )

And by the way, I am agreeing with you. Try not to misunderstand anymore users from now on and save everyone the trouble of dealing with you spewing garbage

 

Sweet. Exactly what I was looking for. Thanks!

@Aesthetic BTW "quick and dirty" is a saying, not to be taken literally. Obviously contamination will equate to mold.

 

Perhaps the only reason he's attacking you is because you didn't really give krozar information but quoted and pointed out the wrong in his post lol. He's here for a reason so instead of saying "wow your new huh?" give him some info to start his own research. There are ways to keep a constant source of mycellium. Check out shroomery.org .... They may not be the best ways but they will work. Krozar im assuming your already in the process of growing, how's it coming along?

 

You cannot get infinite mushrooms from a tissue culture (and noone ever said you could) because over several generations the fungi will lose vigor and get weak forcing you to go back to multispore to rejuvenate the strain but you can inocculate alot of brf/grain/liquid cultures jars for several generations and get a ton of mushrooms.

And there is no reason that you could not use a colonized piece of brf cake to innoculate a liquid culture as long as the cake is 100% clean (no minor contamination). This is why I have said I don't recommend liquid culture because the potential for hidden contamination is larger than that with agar. More often then not you won't know your culture is dirty until you have already innoculated a bunch of jars that are now moldy thus putting you back to square one.